Bis(2-Ethylhexyl) Terephthalate from Olax Imbricata, Natural Substance or Artifact?

The medicinal plant Olax imbricata is well-known for its antioxidant, anti-inflammatory, antibacterial, and diabetes-treating properties. It essential to investigate the chemical composition biological activity of imbricata. During our investigation this plant, a terephthalate derivative, bis(2-ethylhexyl)terephthalate, was isolated from methanol extract using chromatographic techniques elucidated nuclear magnetic resonance spectroscopy. Terephthalate's presence in has raised concerns regarding whether or not it natural substance an artifact. challenging persuade others that bis(2-ethylhexyl)terephthalate biosynthetic compound produced by plant. Sequestration may be result laboratory equipment, adsorbents, solvents used during extraction isolation. This finding cautions staff when plastic utensils exposed organic solvents. A sign identifying silica gel thin layer-chromatography performance eluting with n-hexane : ethyl acetate (96:4). dark spot appears at Rf 0.46 under ultra-violet light 254 nm as steel blue stained solution containing 1% vanillin 10% H2SO4 ethanol.